山羊繁殖季节性相关基因KiSS-1与RFRP的表达研究

doi:10.11843/j.issn.0366-6964.2015.06.007

Abstract

Abstract:

To elucidate the roles of KiSS-1 and RFRP genes in goat，their expression profilings in tissues and the expression levels in hypothalamus-pituitary-ovary (and uterus) were determined between Jining Grey goat (year-round estrus) and Liaoning Cashmere goat (seasonal estrus) by using RT-PCR and qRT-PCR methods，respectively.The results showed that：(i) KiSS-1 mRNA was detected in goat hypothalamus，pituitary，pineal，kidney，ovary，subcutaneous fat and thyroid，while RFRP mRNA was detected in goat hypothalamus，cerebral cortex，cerebellum，ovary and pituitary，both of them had different tissue expression profilings between two goat breeds；(ii) KiSS-1 mRNA expression level was higher in hypothalamus for Jining Grey than that in Liaoning Cashmere goats (P<0.05)，while the expression had no significant difference in ovary and pituitary between 2 breeds (P>0.05)；(iii) The expression level of RFRP mRNA was higher in hypothalamus of Jining Grey than that in Liaoning Cashmere goat (P<0.05)，while its expression in ovary and pituitary was opposite between 2 breeds (P<0.05).The present study suggests that KiSS-1 gene may involve in regulating seasonal breeding in goats，but the effect (or the acting manner) of RFRP gene in goat seasonal breeding need be further researched.The results lay the foundation for elucidating the function of goat KiSS-1 and RFRP genes and provide reference for revealing the genetic mechanism of reproductive seasonality in goat.

CLC Number:

S827
S813.3

HUANG Dong-wei，CHU Ming-xing，DI Ran，LIU Qiu-yue，HU Wen-ping，WANG Xiang-yu，PAN Zhang-yuan，GUO Xiao-fei. Study on Expression of KiSS-1 and RFRP Genes Related to Reproductive Seasonality in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(6): 924-931.

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Study on Expression of KiSS-1 and RFRP Genes Related to Reproductive Seasonality in Goats

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